REBASE
NAR Molecular Biology Database Collection entry number 224
Roberts R.J., Vincze T., Posfai J. and Macelis D.
New England Biolabs, 240 County Road, Ipswich, MA 01938-2723, USA
Contact roberts@neb.com
Database Description
REBASE is a comprehensive database of information about restriction enzymes, DNA methyltransferases and related proteins involved in the biological process of restriction-modification. It contains fully referenced information about recognition and cleavage sites, isoschizomers, neoschizomers, commercial availability, methylation sensitivity, crystal and sequence data. Experimentally characterized homing endonucleases are also included. All newly sequenced genomes are analyzed for the presence of putative restriction systems and this data is included within REBASE.
The contents or REBASE may be browsed from the web (http://rebase.neb.com) and selected compilations can be downloaded by ftp (ftp.neb.com). Additionally, monthly updates can be requested via email.
Currently, there are 11,746 enzymes (including subunits) and 8,601 references stored in REBASE. The references are collected from journals, books, patents, as well as unpublished observations.
There are currently 3,805 biochemically or genetically characterized restriction enzymes in REBASE and of the 3,698 Type II restriction enzymes, 611 are commercially available, including 262 distinct specificities.
Among the 2,709 restriction enzyme genes and 4,485 DNA methyltransferase genes that can be identified in GenBank, the sequenced microbial genomes now account for 3,180 of these genes!
The number of sequenced bacterial and archaeal genomes has risen to more than 400 and the total number of RM systems in REBASE is now almost 3,800 well-characterized systems and more than 4,000 systems, whose existence is predicted on the basis of a bioinformatic analysis of DNA sequences in GenBank.
The contents or REBASE may be browsed from the web (http://rebase.neb.com) and selected compilations can be downloaded by ftp (ftp.neb.com). Additionally, monthly updates can be requested via email.
Currently, there are 11,746 enzymes (including subunits) and 8,601 references stored in REBASE. The references are collected from journals, books, patents, as well as unpublished observations.
There are currently 3,805 biochemically or genetically characterized restriction enzymes in REBASE and of the 3,698 Type II restriction enzymes, 611 are commercially available, including 262 distinct specificities.
Among the 2,709 restriction enzyme genes and 4,485 DNA methyltransferase genes that can be identified in GenBank, the sequenced microbial genomes now account for 3,180 of these genes!
The number of sequenced bacterial and archaeal genomes has risen to more than 400 and the total number of RM systems in REBASE is now almost 3,800 well-characterized systems and more than 4,000 systems, whose existence is predicted on the basis of a bioinformatic analysis of DNA sequences in GenBank.
Category: Protein sequence databases
Subcategory: Databases of individual protein families
Go to the abstract in the NAR 2007 Database Issue.
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